Characterisation of Streptomyces sp. VNUA116 with strong antifungal activity against Fusarium oxysporum f.sp. cubense tropical race 4

Abstract

Banana (Musa spp.) is one of the most important crops in the world. However, banana production worldwide is threatened by the wilt disease caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4). Controlling this disease remains a challenge with conventional control methods, and scientists are searching for long-term, effective, and sustainable strategies. Streptomyces species are known as resources for producing bioactive compounds that represent promising bioagents to control soil-borne diseases. Here, we reported the characteristics of a Streptomyces strain, VNUA116, with strong antifungal activity against Foc TR4. The culture filtrate of the strain VNUA116 exhibited antifungal activities against Foc TR4 by inhibiting mycelial growth and spore germination. Interestingly, the strain VNUA116 also exhibited broad-spectrum antifungal activity against various common pathogenic fungi. Based on the morphological, biochemical, and molecular properties, the strain VNUA116 was identified as Streptomyces sp. In addition, Streptomyces sp.VNUA116 showed valuable productions of IAA, siderophore, and several hydrolytic enzyme activities. Moreover, the presence of the PKS-I and PKS-II genes in the genome of Streptomyces sp. VNUA116 suggests potential for the biosynthesis of bioactive substances. Our finding suggested that Streptomyces sp. VNUA116 could be a potential bioagent for controlling Fusarium wilt and other fungal diseases in bananas.

Keywords:

• Streptomyces sp.
• biocontrol
• Fusarium oxysporum f. sp. cubense
• pathogenic fungi
• antifungal activity

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This work was supported by the Ministry of Science and Technology (MOST), Vietnam under Grant No: NVQG-2021/ĐT.01; Nguyen Thi Thanh Mai was funded by the Master, PhD Scholarship Programme of Vingroup Innovation Foundation (VINIF), code VINIF.2023.TS.085.