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Abstract
This paper presents the results of 3 year‐long monitoring of crop fields in four ecologically different areas of Ukraine for several phytoviral infections. Various modifications of ELISA were used as main assay to evaluate the spreading of a virus. The technique allowing to determine the frequency of occurrence for a virus of interest is proposed. This technique is based on an identification of viral antigens in crops, accompanying weeds and soil. The differences between the investigated regions on a virus occurrence frequency as well as dynamics of the parameter in relation to crop‐rotation have been revealed. The possibility to use results obtained for predicting phytoviral disease spreading is discussed.