In Vitro effects of various xenobiotics on Azotobacter chroococcum strains isolated from soils of southern Poland

Abstract

Fourteen Azotobacter chroococcum strains isolated from soils of Southern Poland were studied concerning resistance to various xenobiotics: heavy metal ions: Cd^2+, Cu²⁺, Fe³⁺, Mn²⁺, Pb²⁺, Zn²⁺, pesticides: herbicides linuron (3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea) and combination of mecoprop ((RS)-2-(4-chloro-2-methylphenoxy)propanoic acid), dicamba (3,6-dichloro-2-methoxybenzoic acid) and MCPA (2-methyl-4-chlorophenoxyacetic acid), fungicide copper oxychloride, insecticide fenitrothion (O,O-Dimethyl O-(3-methyl-4-nitrophenyl) phosphorothioate) and eight antibiotics commonly used against Gram-negative bacteria. The tested soils were divided into seven groups of land use: forest, field crop, park, urban lawn, industrial area, garden and fallow land, and were analyzed for the following heavy metal ion concentrations using the atomic absorption spectrometry (AAS) technique: Cd^2+, Cu²⁺, Fe³⁺, Mn²⁺, Pb²⁺, Zn²⁺. All strains were resistant to Pb²⁺, whereas other metals caused the growth inhibition of the analyzed strains. There was no significant relationship between metal concentrations in the analyzed soils and metal resistance of the isolates. Herbicide linuron did not inhibit the growth of A. chroococcum in any of the concentrations. All other pesticides caused the growth inhibition only in the concentrate forms. All isolates were sensitive to β-lactam antibiotic Meropenem, however high intraspecies differentiation was observed concerning resistance to other antibiotics. The obtained results require further study regarding resistance mechanisms and possible use of the xenobiotic-resistant strains in land rehabilitation.

Keywords:

• Azotobacter chroococcum, xenobiotics, heavy metals, antibiotics, pesticides

Acknowledgments

The analyses were performed within a research project financed by the Ministry of Science and Higher Education no. N N310 081836