Comparison of homologous and heterologous formats in nanocolloidal gold-based immunoassays for parathion residue determination

Abstract

The effectiveness of homologous and heterologous formats in a nanocolloidal gold-based immunoassay for pesticide residue determination was investigated. Parathion, one of the most toxic organophosphorus pesticides, was used as the target analyte. One-step homologous and heterologous test strips based on a nanocolloidal gold-labeled monoclonal antibody were developed for the rapid detection of parathion residues. The results showed that the heterologous format was more effective than the homologous format, being more sensitive, more specific to parathion and more tolerant of matrix interferences. The best competitive hapten was found to have a moderate heterology and the opposite electronic distribution to the immunizing hapten. The detection limits for parathion using the preferred heterologous strip were 1 μg/L in water samples and 5 μg/kg in soil and food samples.

Keywords:

• Nanocolloidal gold
• lateral-flow strip assay
• parathion
• reaction format

Acknowledgment

This work was supported by the Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-year Plan Period (NO2009BADB9B09).