![Sample our Engineering & Technology journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5933/TOC-Subject-Sample-2021-Engineering-Tech.jpg"})
ABSTRACT
The development of field-based biomarkers can allow for a more reliable assessment of the exposure of organisms to pollutants. Different sampling sites, along two streams running through an irrigable agricultural area, were selected to evaluate the effect of agrochemical load on the measured endpoints. The levels of lipid peroxidation were evaluated in several organs of Procambarus clarkii. The same method was applied to leaves of two woody species. Determining levels of MDA (malonaldehyde) by thiobarbituric acid reactive substances assay allows measuring the levels of lipid peroxidation. Differences in lipid peroxidation levels were observed in P. clarkii individuals collected at different sites; however, the patterns varied depending on the organ (when accounting for variations due to sex). The use of a MDA-gills/MDA-hepatopancreas index allowed for discrimination between reference and polluted sites. Significant differences in oxidative damage between sites were found in the leaves of Quercus rotundifolia but not in Salix sp. The lipid peroxidation of crayfish organs and holm oak leaves as a suitable biomarker of environmental pollution deserves further investigation.
Abbreviations
| BHT | = | 2,6-di-tert-buthyl-4-methy-lphenol |
| CGMs | = | Models of oceanic atmospheric circulation |
| MDA | = | Malondialdehyde |
| PNR | = | Spanish National Irrigation Plan |
| POPs | = | Persistent Organic Pollutants |
| ROS | = | Reactive oxygen species |
| TBA | = | Thiobarbituric acid |
| TBARS | = | Thioarbituric acid reactive substances |
| TEP | = | 1,1,3,3-tetramethoxypropane |
| WFD | = | Water Framework Directive |
Authors contributions
The study was designed by J. San Sebastian Sauto and M. Catalá. The sampling and field work was performed by J.L. Rodríguez-Gil, P. Sánchez Sánchez, J. San Sebastián Sauto and M. Catalá. The analysis of the samples was carried out by J.L. Rodríguez-Gil. The article was written by M.R. de las Heras assisted by J.L. Rodríguez-Gil under the direction of M. Catalá. All the authors have read and approved the paper.
Acknowledgments
This work has been funded by SEPI and TRAGSA Group within BIOINRIE 30–13.436 R&D Project. We wish to thank Dr. S. González-Alonso for her help during field work and sampling.