Assessment of environmental quality of water bodies next to agricultural areas of Buenos Aires province (Argentina) by means of ecotoxicological studies with Rhinella arenarum

Abstract

Little is known about the effects of polluted water bodies from Buenos Aires Province on the development of native fauna. Ecotoxicological quality of water bodies from agricultural sites was evaluated by means of standardized laboratory bioassays with embryos and larvae of the native amphibian Rhinella arenarum. The organisms were acutely and chronically exposed to surface water samples from streams of Arrecifes (A), Pergamino (P) and Salto (S) districts that represent the most important agricultural core from the region. Lethal, sublethal and genotoxic effects were assessed. Water sample from (A) caused chronic toxicity (LC₅₀:45.35%) in embryos, followed by (S) and the water sample from (P) was not toxic. In larvae, an inversion of the toxicity pattern was found. Thus, the 504 h-LC₅₀s were 28.12%, 39% and 61% for (S), (P) and (A), respectively. A stage-dependent sensitivity was registered, being larvae more affected than embryos. Significant genotoxic effects, estimated by micronucleus test were observed in the larvae exposed to water samples from all sites. The present study warns about environmental degradation of surface waters next to agricultural areas of Buenos Aires Province. This fact jeopardizes R. arenarum populations in this area.

Keywords:

• Agricultural pollution
• amphibians
• bioassays
• genotoxicity
• water quality

Additional information

Funding

This study was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) PIP 112 201301 00140 CO research grant. We thank Ferring Pharmaceuticals for providing the human chorionic gonadotropin. We also thank Centro de Investigaciones del Medioambiente (CIM), Facultad de Ciencias Exactas, UNLP, for their help in the water sample collection and to the memory of Alicia Ronco.