Plasma cholinesterase activity is influenced by interactive effect between omethoate exposure and CYP2E1 polymorphisms

Abstract

The aim of this study was to explore the association between metabolizing enzyme gene polymorphisms and the decrease in cholinesterase activity induced by omethoate exposure. A total of 180 workers exposed to omethoate over an extended period were recruited along with 115 healthy controls. Cholinesterase activity in whole blood, erythrocyte, and plasma was detected using acetylthiocholine and the dithio-bis-(nitrobenzoic acid) method. Six polymorphic loci of GSTT1(+/-), GSTM1(+/-), GSTP1 rs1695, CYP2E1 rs6413432, CYP2E1 rs3813867, and PON2 rs12026 were detected by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The gene-environment interactions were analyzed using the generalized linear model method. The cholinesterase activity of erythrocyte and plasma in the exposure group was significantly lower than that in the control group (P < 0.001) in general. The plasma cholinesterase activity in the TT + AT genotype in CYP2E1 rs6413432 was lower than that in the AA genotype in the exposure group (P = 0.016). Interaction between the AA genotype in CYP2E1 rs6413432 and omethoate exposure had a significant effect on plasma cholinesterase activity (P = 0.079). The decrease in plasma cholinesterase activity was associated with interaction between the AA genotypes in rs6413432 and omethoate exposure.

Keywords:

• Omethoate
• cholinesterase activity
• CYP2E1
• polymorphism

Acknowledgments

Dr. Wei Wang designed the study and applied for Research Ethics Board approval. Tuanwei Wang and Lei Li recruited the patients and collected the data. Hui Zhang analyzed the data and prepared draft figures and tables. Hui Zhang and Dr. Wenjuan Zhang and Qi Wang prepared the manuscript draft with important intellectual input from Dr. Wei Wang and Tuanwei Wang. All authors approved the final manuscript. All authors had complete access to the study data.

Conflict of interests

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by grants from Outstanding Young Scholar of Double Hundred Talents of Jinan University and National Natural Science Foundation of China (No. 81473014; W Zhang), Outstanding Youth Grant of Zhengzhou University (1521329035) and Programs for Science and Technology Development of Zhengzhou (131PPTGG376).