Development of a sensitive chicken IgY-based enzyme-linked immunosorbent assay for detection of mebendazole in pork and mutton

Abstract

Chicken egg yolk IgY has proven to be qualified for analysis of targets in immunoassays. In order to explore the feasibility of chicken IgY-based ELISA for detection of mebendazole (MEB), the chicken IgY against MEB was generated in the laying hens. An enzyme-linked immunosorbent assay (ELISA) based on chicken IgY was developed for detection of MEB with a half-maximum signal inhibition concentration (IC₅₀) of 3.65 ng mL⁻¹ and a limit of detection of 0.25 ng mL⁻¹. The assay showed a lower cross reactivity (less than 1%) with other structures analogues (except amino-MEB with the values of 70.7%). The average recoveries of MEB spiked in pork and mutton muscle samples ranged from 93.6% to 106.3% with relative standard deviation less than 8.78% and 10.85% for intra-assay and inter-assay, respectively, and agreed well with those of high-performance liquid chromatography. Our results indicate that generated IgY could be used as a robust reagent for routine screening analysis of small molecular compounds residues in food samples.

Keywords:

• Mebendazole
• chicken IgY
• ELISA
• pork
• mutton

Data availability statement

The data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of interest

The authors declare that they have no conflict of interest.

Additional information

Funding

This study was supported by the Project of Scientific Research for Excellent Doctors (SXYBKY2019026), Shanxi Province, China. Project of Science and Technology Innovation for Universities (2020L0164), Shanxi Province, China. Project of Science and Technology Innovation Fund of Shanxi Agricultural University (2020BQ45). Innovation Projects of College of Veterinary Medicine, Shanxi Agricultural University (DYXY2021DC08; DY-Q012). Fund for Shanxi “1331 Project” (20211331-13).