Optimization and validation of LLE-LTP to determine florpyrauxifen-benzyl herbicide in water samples by HPLC-DAD

Abstract

Florpyrauxifen-benzyl is a systemic herbicide which acts on weeds of common occurrence in rice cultivation areas using flooded or rainfed systems. The Brazilian Health Regulatory Agency (ANVISA) authorized the commercialization of this pesticide, but did not establish guidelines with an extraction and quantification method of residues of this compound as a form of environmental monitoring. Therefore, the present study aimed to optimize and validate the liquid-liquid extraction with low temperature purification (LLE-LTP) to determine florpyrauxifen-benzyl content in water samples by high performance liquid chromatography with diode array detection (HPLC-DAD). The recovery ranged from 95.84 to 105.4% with a relative standard deviation less than 1.5. LLE-LTP was selective, precise, accurate, linear in the range from 4.00 to 150 µg L⁻¹, and the limit of quantification was 4.00 µg L⁻¹. The stability study of the compound in water revealed its degradation in 25 days and DT₅₀ in approximately 5 days. LLE-LTP coupled to HPLC-DAD presented itself as a simple, easy and efficient method of extracting and analyzing it in water samples.

Keywords:

• Arylpicolinate herbicide
• florpyrauxifen-benzyl
• LLE-LTP
• sample preparation
• synthetic auxins herbicide

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article [and/or] its supplementary materials.

Additional information

Funding

The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) [308480/2019-8] for the financial support from research fellowships. The authors are also grateful to the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) [APQ-02736-21], Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Universidade Federal de Minas Gerais (UFMG), for the infrastructure provided.