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Abstract
The characteristics of a series of natural pH gradients, generated in layers of granulated gels using mixtures of amphoteric or amphoteric-non-amphoteric buffer reagents, are described. Because of the favourable properties of these flat bed systems, high voltage gradients (20–50V/cm) can be used for the electrofocusing experiments. Linear or step pH gradients can be formed depending on the choice of buffer system used. Buffer systems, suitable for the preparative electrofocusing of proteins over the PI ranges of 4.0–6.0, 4.5–8.0, 5.0–8.5 and 6.0–8.5 are reported. In contrast to results obtained with polyacrylamide gel supports, non amphoteric buffers form very steep and unstable pH gradients on beds of granulated gels.