Surface Plasmon Resonance-Based Molecular Detection of Hb S [β6(A3)Glu→Val, GAG→GTG] at the Gene Level

Abstract

The surface plasmon resonance (SPR) approach, being a relatively novel biophysical method, is used to detect many different targets by biomolecular interaction. The SPR system uses optical and evanescent wave phenomenon. This approach does not need any labels, such as enzymes or isotopes, and the monitored interactions are in real time. In DNA-DNA interaction, the SPR approach is Tm-independent. Here we report our preliminary results for the molecular detection of the Hb S (GAG →GTG) mutation at codon 6 of the human β-globin gene. Our preliminary results show that the SPR approach could be applied as an inexpensive and fast routine test system for the molecular diagnosis of abnormal hemoglobins (Hbs), especially in premarital screening programs.

Keywords:

• Surface plasmon resonance (SPR)
• Hb S
• Abnormal hemoglobin (Hb)
• Mutation detection

Notes

10. IASys Methods Guide, Affinity Sensors, 2nd ed. Cambridge, 1996.