Effect of different fertilizers on the bacterial community diversity in rhizosperic soil of broomcorn millet (Panicum miliaceum L.)

ABSTRACT

The bacterial community structure and diversity in the rhizosphere of broomcorn millet treated with different fertilizers were analyzed. The crops were grown under one of the four fertilization treatments: M1 (compound fertilizer), M2 (farmyard manure), M3 (biological bacterial fertilizer), and M4 (no fertilizer). The soil DNA was isolated, and the 16S rRNA library was sequenced using Illumina MiSeq sequencers. The bacterial diversity and the differential bacterial genera in four groups were analyzed. Compound fertilizers had the most significant effect on plant growth, followed by farmyard manure and then biological bacterial fertilizer (p < 0.05). Actinobacteria and Pseudomonas sp. were dominant in all soil samples. The abundance of Actinobacteria was higher in M1 and M4, and the abundance of Pseudomonas sp. was higher in M3 and M4. The number of bacterial genera (208) was the highest in M1. Eight bacterial genera with low abundance were common in M1 vs. M3 and M2 vs. M3. Four bacterial genera with high richness (Phormidium sp., Cellulosimicrobium sp., Yaniella sp., and Cloacibacterium sp.) overlapped in M1 vs. M3 and in M2 vs. M3. Compound fertilizer was the most effective at encouraging broomcorn millet growth (plant height, stem diameter, panicle weight, grain weight, and yield).

KEYWORDS:

• Broomcorn millet
• rhizosphere soil
• fertilizer management
• diversity of bacterial communities
• differential bacterial community

Availability of data and materials

The raw sequencing data have been deposited in NCBI SRA (Sequence ReadArchive) database with the accession number of SRP225955 (https://dataview.ncbi.nlm.nih.gov/object/PRJNA577810?reviewer=v8p0sqo1l8t32d87a1dsso68ob).

Disclosure statement

The authors declare that they have no competing interests.

Ethics approval and consent to participate

This study was approved by the Ethics Committee of Shanxi Agricultural University and Shanxi University.

Additional information

Funding

This work was supported by Postdoctoral Science Foundation (Program No. YCX2018D2BH3), the earmarked fund for China Agriculture Research System (Program No. CARS-06-13.5-A16), Shanxi Provincial Key Research and Development Program (Program No. 201803D221020-6), National Natural Science Foundation of China (Program No. Nos. 31760612), Shanxi National Academy of Agricultural Sciences National Natural Fund Support and Cultivation Project (Program No. YGJPY2001), the earmarked fund for Modern Agro-industry Technology Research System (2020-03) and Natural Science Foundation of Shanxi Province (Program No. 201601D102049).