Abstract
Objective In order to investigate multilineage differentiation in human cultured cells from ethmoid bone, we conducted a morphological study to examine adipogenic and chondrogenic differentiation.
Material and Methods After reaching confluence, cells underwent terminal adipogenic differentiation by treatment with 100 μM indomethacin, 0.5 mM 1-methyl-3-isobutylxanthine, 1 μM dexamethasone (DEX), 10 μg/ml insulin and 0.3% dimethylsulfoxide in a medium supplemented with 10% fetal bovine serum. Chondrogenic differentiation was attempted by centrifuging a pelleted micromass using transforming growth factor-β3 (TGF-β3), DEX, ascorbic acid (AA), pyruvate acid, proline, glucose and (ITS)-plus.
Results The cultured cells displayed adipocyte but not chondrogenic lineage under these conditions. Considering the possibility that some differentiation potential may be lost with in vitro culture but maintained using another chondrogenic differentiation medium containing TGF-β1, it is possible that cultured cells may have multilineage potential, including chondrogenic differentiation ability.
Conclusions These morphological abilities of human cultured cells may indicate the possibility of the existence of mesenchymal stem cells in sinus bone. If mesenchymal stem cells exist in ethmoid bone, they may play an important role in future research on the regulation mechanisms of human bone tissue.
Ishino T, Hirakawa K, Takeno S, Furukido K, Sugimoto I, Yajin K. Bone-constructing cells from ethmoid bone may have multilineage differentiation potential: preliminary report. Acta Otolaryngol 2004; Suppl 553: 105–108.
Ishino T, Hirakawa K, Takeno S, Furukido K, Sugimoto I, Yajin K. Bone-constructing cells from ethmoid bone may have multilineage differentiation potential: preliminary report. Acta Otolaryngol 2004; Suppl 553: 105–108.