Fast-green staining of interphase nucleoli in lemon fruit explants

Summary

Interphase nucleoli of freshly excised lemon fruit tissue showed a weak staining reaction with alkaline fast-green compared with that of enlarged interphase nucleoli of 48-hour-old tissue explants. Fast-green staining bodies were seen at various regions along the chromatin strands of the interphase nuclei of freshly excised tissue but absent from those of the 48-hour-old explants where nucleolar enlargement had occurred. The changes following tissue explanation are considered to indicate activation of the genome in response to the new environment.