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Abstract
The oxidative degradation of glucose by coli-aerogenes bacteria was investigated: a remarkable yield (more than 0.5 mole per one mole of glucose) of α-ketoglutaric acid was obtained with suspensions of washed cells of the bacteria grown aerobically on glucose medium, and pyruvic acid fermentation was revealed in the presence of arsenic compounds not only with growing culture but also with the washed cells. Moreover, pyruvic acid was found to be a potent precursor of α-ketoglutaric acid.
Abstract
The production of α-ketoglutaric acid by the bacteria of coli-aerogenes was observed to be inhibited in the presence of αα'-dipyridyl or ο-phenanthroline. From experimental results it was concluded that iron might be a sort of cofactor of the enzyme system producing α-ketoglutaric acid. Moreover, α-ketoglutaric acid was found to be produced not only from lactic acid by either growing culture or the washed cells, but also from pyruvic acid under enzymatic conditions such as dried cells or cell-free extracts.