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Laboratory tests on Brachionus calyciflorus Pallas indicate that at low concentrations of free ammonia (< 7 mg l −1) pH levels up to 9,85 could be tolerated for 96 h without apparent ill effect. Free ammonia concentrations > 14,4 mg l −1 (as N) could not be tolerated by this species. It is recommended that ammonium hydroxide be added to HRAP's in order to elevate the concentration of free ammonia above this level.
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