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Abstract
A dot-blot hybridization test was used to survey for the presence of Potato spindle tuber viroid in seed potatoes produced in the Canadian provinces of British Columbia, Alberta, and Saskatchewan. Four hundred leaves were collected randomly from 201 and 225 fields of seed potatoes in 1999 and 2000, respectively. Leaves were tested in composites of 100 by extracting the nucleic acid fraction and probing it with a digoxigenin-labeled cRNA probe. RNA duplexes were detected using an antibody-based colorimetric detection assay. All test samples were negative corroborating visual inspection observations that spindle tuber does not occur in seed potatoes in these provinces.