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Disease report/Rapport des maladies

First report of Stemphylium lycopersici causing leaf spot on hot pepper in China

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Pages 124-128 | Accepted 22 Aug 2018, Published online: 23 Jan 2019
 

Abstract

Hot pepper is an exceedingly popular vegetable crop in China and is cultivated on 737 hectares in Beijing. A severe leaf disease with typical symptoms of spots with grey centres and dark brown borders was observed on hot pepper plants in Beijing in the winter of 2016. More than 50% of all plants in the region were infected. Fungal cultures were isolated from naturally infected leaf tissue, and identified as Stemphylium lycopersici based on morphological features, cultural characteristics and molecular identification by sequencing the ITS, gpd and cmdA genes. Pathogenicity was determined by inoculating healthy pepper plants with hyphal suspensions, and the fungus was re-isolated from developing lesions on the inoculated plants, thus fulfilling Koch’s postulates. This is the first report of natural infection by S. lycopersici causing leaf spot on hot pepper in China.

Résumé

Le piment fort est un légume extrêmement populaire en Chine et, à Beijing, on consacre 737 ha à sa culture. Une grave maladie foliaire, affichant les symptômes typiques de taches aux centres gris et aux contours bruns, a été observée sur les plants de piments forts à Beijing durant l’hiver de 2016. Plus de 50% de tous les plants de la région étaient infectés. Des cultures fongiques ont été isolées à partir de tissus foliaires infectés naturellement et, en se basant sur les traits morphologiques, les caractéristiques culturales et l’identification moléculaire en séquençant l’ITS ainsi que les gènes gpd et cmdA, l’agent a été identifié en tant que Stemphylium lycopersici. La pathogénicité a été déterminée en inoculant des plants de piments sains avec des hyphes en suspension, puis le champignon a été de nouveau isolé à partir des lésions qui s’étaient développées sur ces derniers, confirmant ainsi les postulats de Koch. Il s’agit de la première mention d’une infection naturelle provoquée par S. lycopersici, causant des taches foliaires sur le piment fort en Chine.

Additional information

Funding

This work was supported by CAAS, the Science and Technology Innovation Program, and funded by the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, China; the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, China..

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