The Determination of Brain Magnesium and Zinc Levels by a Dual-Probe Microdialysis and Graphite Furnace Atomic Absorption Spectrometry

Dar-Yu Yang Department of Emergency (D.Y.Y.), Kaoshiung Medical University, Kaoshiung, TAIWAN

, MD, MSC,

Jen-Bin Lee Medical Research (J.B.L., Y.J.L., F.C.C.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Taichung Veterans General Hospital, Department of Applied Chemistry, Providence University (J.B.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN

, MS,

Ming-Cheng Lin Department of Medical Technology, Chung-Tai Institute of Health Sciences and Technology (M.C.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Graduate Institute of Medicine, (M.C.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN

, MS,

Yeou-Lih Huang School of Technology for Medical Sciences (Y.L.H.), Kaoshiung Medical University, Kaoshiung, TAIWAN

, PhD,

Hong-Wen Liu Department of Emergency (D.Y.Y.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Medical Research (J.B.L., Y.J.L., F.C.C.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Taichung Veterans General Hospital, Department of Applied Chemistry, Providence University (J.B.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Department of Medical Technology, Chung-Tai Institute of Health Sciences and Technology (M.C.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN; Graduate Institute of Medicine, (M.C.L.), Kaoshiung Medical University, Kaoshiung, TAIWAN; School of Technology for Medical Sciences (Y.L.H.), Kaoshiung Medical University, Kaoshiung, TAIWAN

, MD,

Yea-Jiuan Liang Medical Research (J.B.L., Y.J.L., F.C.C.), Kaoshiung Medical University, Kaoshiung, TAIWAN

, MS &

Fu-Chou Cheng Medical Research (J.B.L., Y.J.L., F.C.C.), Kaoshiung Medical University, Kaoshiung, TAIWANCorrespondence[email protected]

, PhD show all

Abstract

Objective: The aim of this study was to develop a microdialysis-graphite furnace atomic absorption spectroscopy (MD-GFAAS) for monitoring dynamic changes of extracellular magnesium (Mg) and zinc (Zn) in the cortex of gerbils subjected to focal cerebral ischemia, that had been produced in anesthetized gerbils by occlusion of the right middle cerebral artery.

Methods: Two microdialysis probes were inserted into both sides of the cortex to simultaneously collect dialysates during cerebral ischemia. Dynamic changes in these analytes, on ipsilateral and contralateral sides of the brain, were assayed by MD-GFAAS. Optimal conditions and analytical precision of GFAAS were studied in the present assay.

Results: The present study demonstrated significant decreases in Mg (65% of baseline) and zinc (74% of baseline) maintained their levels within 3 h on the ipsilateral side of cortex during cerebral ischemia. Slight changes of Mg and Zn on the contralateral sides were also observed.

Conclusion: The derangement of extracellular Mg and Zn could be important in the progression of cell injury and may be associated with cerebral ischemia insult.

Keywords

This study was supported by grants from the Taichung Veterans General Hospital (TCVGH-927207C & 927316D) Taiwan, Republic of China.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

The Determination of Brain Magnesium and Zinc Levels by a Dual-Probe Microdialysis and Graphite Furnace Atomic Absorption Spectrometry

Information for

Open access

Opportunities

Help and information

The Determination of Brain Magnesium and Zinc Levels by a Dual-Probe Microdialysis and Graphite Furnace Atomic Absorption Spectrometry

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature