Characterization of A Glycosylphosphatidylinositol- Anchor in a Cell Adhesion Molecule, Csa, from Dictyostelium Discoideum¹

1. Presented at the XVIII th Intenrarional Carbohydrate Symposium, Milan, Italy, July 21-26, 1996.

Abstract

The cell adhesion molecule, csA, treated with lysyl endopeptidase led to a peptide fragment with a molecular weight of 31 kDa (31-kDa csA). It was shown after the treatment of the 31-kDa csA with phosphatidylinositol-specific phospholipase C and following extraction with chloroform using TLC that the 31-kDa csA contained a component of glycosylphosphatidylinositol (GPI), and it was also confirmed that the cell adhesion molecule, csA, consisted of ceramide instead of diacylglycerol. The results from GC-MS suggested that hydrocarbons of C₁₆- C₃₃ interacted with the GPI-anchor region.

1. Presented at the XVIII th Intenrarional Carbohydrate Symposium, Milan, Italy, July 21-26, 1996.

Notes

1. Presented at the XVIII th Intenrarional Carbohydrate Symposium, Milan, Italy, July 21-26, 1996.