Abstract
The detailed study of internucleotidic bond formation by the use of bis(2,4,6-tribromophenyl) phosphorochloridate (TBP) is described. The 31P NMR analysis suggested that the TBP-mediated condensation proceeded via a reactive unsymmetrical pyrophosphate intermediate, which was readily activated in the presence of 3-nitro-1,2,4-triazole to form internucleotidic bond.