Elucidation of the Mechanism by Which 9-(Trans-2′, Trans-3′-Dihydroxycyclopent-4′-enyl)-Adenine Lnactivates S-Adenosylhomocysteine Hydrolase and Elevates Cellular Levels of Sadenosylhomocysteinew

Abstract

Evidence is presented that 9-(trans-2′, trans-3′-dihydroxycyclopent-4′-enyl)-adenine (1) is oxidized to the 3′-ketonucleoside 2 with concomitant reduction of NAD⁺ to NADH by S-adenosylhomcysteine hydrolase. We also describe how the carbocyclic nucleoside I has been used to reveal the metabolic relationships between Sadenosylmethionine, S-adenosylhomocysteine and homocystcine in murine L-929 cells.