Large Scale HPLC Purification of Oligodeoxyribonucleotides for Studies of the Binding of CC-1065 to DNA by NMR Spectroscopy

S. P. Pulaski Biopolymer Chemistry and The Upjohn Company, Kalamazoo, Mi, 49001

N. Theriault Hatzenbuhler Biopolymer Chemistry and The Upjohn Company, Kalamazoo, Mi, 49001

T. A. Scahill Physical and Analytical Chemistry, The Upjohn Company, Kalamazoo, MI, 49001

N. Richter Physical and Analytical Chemistry, The Upjohn Company, Kalamazoo, MI, 49001

Abstract

We have investigated four different HPLC columns and several conditions in search of the most efficient procedure for purification of milligram amounts of DNA. We are reporting the optimal conditions for a preparative purification using a Dupont Zorbax Bioseries Oligo column.

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Large Scale HPLC Purification of Oligodeoxyribonucleotides for Studies of the Binding of CC-1065 to DNA by NMR Spectroscopy

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Large Scale HPLC Purification of Oligodeoxyribonucleotides for Studies of the Binding of CC-1065 to DNA by NMR Spectroscopy

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