Studies on the Formation of the Internucleotidic Bond in RNA Synthesis Using Dialkylamino Phosphoroamidites

D. Gasparutto Laboratoires de Chimie. Departement de Recherche Fondamentale. Centre d'Etudes Nucleates de Grenoble, 85 X - 38041, Grenoble Cedex, FRANCE

D. Molko Laboratoires de Chimie. Departement de Recherche Fondamentale. Centre d'Etudes Nucleates de Grenoble, 85 X - 38041, Grenoble Cedex, FRANCE

R. Teoule Laboratoires de Chimie. Departement de Recherche Fondamentale. Centre d'Etudes Nucleates de Grenoble, 85 X - 38041, Grenoble Cedex, FRANCE

Abstract

Several N,N-dialkylamlno-cyanoethyl-phosphoroamidites were tested for use in solid-phase synthesis of homo-uridine RNA fragments. N-ethyl-N-me-thylamino protection gave the best results. The corresponding monomer is stable for several months and an average coupling yield of 97 % was obtained after 4 minutes of condensation using this agent.

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Studies on the Formation of the Internucleotidic Bond in RNA Synthesis Using Dialkylamino Phosphoroamidites

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Studies on the Formation of the Internucleotidic Bond in RNA Synthesis Using Dialkylamino Phosphoroamidites

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