Polymorphism, Packing, Resolution, and Reliability in Single-Crystal DNA Oligomer Analyses

Richard E. Dickerson Molecular Biology Institute, University of California, Los Angeles, CA, 90024, U. S. A.

Kazimierz Grzeskowiak Molecular Biology Institute, University of California, Los Angeles, CA, 90024, U. S. A.

Maria Grzeskowiak Molecular Biology Institute, University of California, Los Angeles, CA, 90024, U. S. A.

Mary L. Kopka Molecular Biology Institute, University of California, Los Angeles, CA, 90024, U. S. A.

Teresa Larsen Molecular Biology Institute, University of California, Los Angeles, CA, 90024, U. S. A.

Andrei Lipanov Molecular Biology Institute, University of California, Los Angeles, CA, 90024, U. S. A.

Gilbert G. Privé Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, Building 3, University of California, Berkeley, CA, 94720

Jordi Quintana Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, Building 3, University of California, Berkeley, CA, 94720

Peter Schultze Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, Building 3, University of California, Berkeley, CA, 94720

Kazunori Yanagi Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, Building 3, University of California, Berkeley, CA, 94720

Hanna Yuan Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, Building 3, University of California, Berkeley, CA, 94720

Hyo-Chun Yoon Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, Building 3, University of California, Berkeley, CA, 94720

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Abstract

Synthetic double-helical B-DNA oligonucleotides crystallize in orthorhombic, monoclinic, and trigonal patterns that are determined by fine details of intermolecular contacts. Crystal packing apparently has relatively little influence on local helix structure, and noncrystallographic symmetry differences can be used to evaluate the quality of analyses.

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Polymorphism, Packing, Resolution, and Reliability in Single-Crystal DNA Oligomer Analyses

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Polymorphism, Packing, Resolution, and Reliability in Single-Crystal DNA Oligomer Analyses

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