Synthesis of Oligodeoxynucleotides Bearing a Radiation Induced DNA Damage: Deoxyribosylurea and Deoxyribosylformamide

Abstract

Formamido and ureido residues were introduced in DNA fragments using PAC cyanoethylphosphoramidite methodology. Mild alkaline conditions were used for the deprotection of the oligonucleotides and the integrity of these fragile defects was confirmed by FAB Mass spectrometry.