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3′ -O-(Diisopropylamino-2-cyanoethoxyphosphinyl)-5′ -0-(4, 4′ -dimethoxytrityl)-N2-isobutyryl-8-oxo-2′-deoxy-7H-guanosine was synthesized and used for the introduction of an 8−0×0−2′-deoxy-7H-guanosine residue into a DNA oligomer by means of automated synthesis. A modification of the preparation of the phosphoramidite markedly improves the coupling efficiency in the oligomer synthesis in this and several other cases that were tested.
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