Synthesis of Fused Oligoribonucleotides with Trideoxyribonucleotide Containing Phosphorothioate to Stabilize Against Nuclease Activity

Masahide Ishikawa Department of Industrial Chemistry, Faculty of Engineer-ing, The University of Tokyo, Hongo, Tokyo, 113, Japan

Kazunori Ikebukuro Department of Industrial Chemistry, Faculty of Engineer-ing, The University of Tokyo, Hongo, Tokyo, 113, Japan

Ichiro Hirao Department of Industrial Chemistry, Faculty of Engineer-ing, The University of Tokyo, Hongo, Tokyo, 113, Japan

Kin-Ichiro Miura Department of Industrial Chemistry, Faculty of Engineer-ing, The University of Tokyo, Hongo, Tokyo, 113, Japan

Abstract

Fused oligonucleotides(21mer) consisting of RNA(18mer) and DNA(3mer) were synthesized by combined use of the phosphotriester and phosphoramidite methods. The RNA(18mer) corresponds to the leader sequence of phage fl coat protein mRNA containing initiation codon. The RNA was stabilized against 3′-exonucleases by joining with trideoxy-ribonucleotides containing phosphorothioate linkages and it would be applied to the studies on the initiation complex formation in prokaryotic translation.

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Synthesis of Fused Oligoribonucleotides with Trideoxyribonucleotide Containing Phosphorothioate to Stabilize Against Nuclease Activity

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Synthesis of Fused Oligoribonucleotides with Trideoxyribonucleotide Containing Phosphorothioate to Stabilize Against Nuclease Activity

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