Nucleosides and Nucleotides. 105. DNA Bending in d(A)₄ - d(T)₄ TRACTS Containing 3-Deazaadenine or 7-Deazaadenine Substituted for Adenine^§,1

Abstract

Self-complementary decadeoxyribonucleotides containing 3-deazaadenine or 7-deazaadenine in place of one of the adenine moieties in a parent sequence, d(GAAAATTTTC)_n, have been synthesized. After phosphorylation at their 5′-ends, the decamers were ligated to form multimers, which were analyzed by polyacrylamide gel electrophoresis. The multimers of the decamer containing 3-deazaadenine (3), d(GAA3ATTTTC)_n, showed decreased bending compared with the multimers of d(GAAAATTTTC)_n, while the decamer containing 3-deazaadenine at a different position, d(GAAA3TTTTC)_n, did not show any degree of bending. Also, the properties of migration of the multimers containing 7-deazaadenine in the gel will be discussed.