Recognition of the Nucleoside in the First Position of the Anticodon of Isoleucine tRNA by Isoleucyl-tRNA Synthetase from Escherichia Coli

Tomonari Muramatsu Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113, Japan

Tatsuo Miyazawa Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113, Japan; Protein Engineering Research Institute, Furuedai, Suita, Osaka 565, Japan

Shigeyuki Yokoyama Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113, Japan

Abstract

Escherichia coli tRNA^Ile ₁ prepared in vitro by T7 RNA polymerase transcription was found to be charged with isoleucine by E. coli isoleucyl-tRNA synthetase (IleRS). Replacement of G in the first position of the anticodon with U, C, or A resulted in complete loss of isoleucine-accepting activity. This indicates that IleRS strictly recognizes the structural feature of guanosine in this position of tRNA^Ile ₁ which is common to that of lysidine in E. coli tRNA^Ile ₂.

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Recognition of the Nucleoside in the First Position of the Anticodon of Isoleucine tRNA by Isoleucyl-tRNA Synthetase from Escherichia Coli

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Recognition of the Nucleoside in the First Position of the Anticodon of Isoleucine tRNA by Isoleucyl-tRNA Synthetase from Escherichia Coli

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