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Nucleosides and Nucleotides Volume 11, 1992 - Issue 2-4
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Original Articles

Conformational Rigidity of N4-Acetyl-2′-O-methylcytidine Found in tRNA of Extremely Thermophilic Archaebacteria (Archaea)

Gota Kawai Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113, Japan; Faculty of Engineering, Yokohama National University, Tokiwadai, Hodogaya-ku, Yokohama, 240, Japan
,
Takeshi Hashizume Departments of Medicinal Chemistry and Biochemistry, University of Utah, Salt Lake City, Utah, 84112, U.S.A.
,
Masayuki Yasuda Faculty of Engineering, Yokohama National University, Tokiwadai, Hodogaya-ku, Yokohama, 240, Japan
,
Tatsuo Miyazawa Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113, Japan; Protein Engineering Research Institute, Furuedai, Suita, Osaka, 565, Japan
,
James A. McCloskey Departments of Medicinal Chemistry and Biochemistry, University of Utah, Salt Lake City, Utah, 84112, U.S.A.
&
Shigeyuki Yokoyama Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113, Japan
Pages 759-771 | Received 19 Sep 1991, Accepted 15 Nov 1991, Published online: 23 Sep 2006
 
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Abstract

The conformational characteristics of N 4-acetyl-2′-O-methylcytidine (ac4Cm), which is one of the modified cytidines unique to the tRNA of extremely thermophilic archaebacteria, and related nucleosides, N 4-acetylcytidine (ac4C), 2′-O-methylcytidine (Cm) and cytidine, were analyzed by proton nuclear magnetic resonance spectroscopy. Ribose methylation and N 4-acylation were found to confer high conformational rigidity to the ribose moiety, suggesting that these post-transcriptional modifications play a role in structural stabilization of tRNA, which is of particular importance at high temperature.

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