Abstract
A method has been devised to synthesize a DNA-duplex with covalently connected strands. Primary amino group located on one strand is linked to a carboxyl group of the other strand through the agency of a water soluble carbodiimide condensing agent. Conditions for the reaction between chains of a duplex composed of the modified oligonucleotides [1] were optimized. The thermal and hydrolytic stability of the cross linked duplex was examined.
Notes
Abbreviations used: Prefix “d” (deoxy) is omitted in design of 2′-deoxyribonucleosides and oligodeoxyribonucleotides; Fmoc—fluorenilmethyloxicarbonyl; FITC—fluorescein isothiocyanate; CDI—N′-(3-dimethyl-aminopropyl)-N-ethylcarbodiimid hydrochloride.