Fluorescent-Labeled Oligonucleotide Probes with Non-Nucleotide Linker: Detection of Hybrid Formation by Fluorescence Anisotropy

Abstract

Fluorescein labeled oligonucleotide probes with non-nucleotide linker have been synthesized and used to monitor hybrid formation to detect DNA sequences in solution. Fluorescence anisotropy, r, was adopted as an index to monitor triple helix formation and the behaviour of F-Probe in solution. An appreciable increase in anisotropy was observed upon use of non-nucleotide linker in the fluorescence probe as compared to that of the F-Probe without non-nucleotide linker.