Enzymatic Fluorescence and Biotin Labelling of Primers for Dna Sequencing and Pcr

Abstract

Using terminal deoxynucleotidyltransferase, it is possible to tag post-synthetically a sequencing primer with a fluorescent label. This procedure gives rise to a 3′ terminally labelled oligodeoxynucleotide, elongated by a single labelled ribonucleotide that retains its substrate properties for a variety of DNA polymerases. A 3′ terminal biotin molecule may be incorporated into a PCR primers analogously.