Synthetic Oligonucleotide Combinatorlal Libraries - Tools For Studying Nucleic Acid Interactions

Abstract

This paper gives an overview of methods available for, and difficulties encountered in the preparation of synthetic oligonucleotide combinatorial libraries. The application of libraries immobilized to high-load beads to the screening of specific two-component (nucleic acid / nucleic acid) and three-component (ds nucleic acidprotein) interactions is described. The effector oligonucleotides immobilized to a single bead are identified by direct Maxam-Gilbert sequencing.