In Vitro Selection, Structural Characteristics, and Photocleavage of an Isoalloxazine Binding Rna Aptamer

Abstract

ABSTRACT: An RNA motif which binds FMN was isolated by in vitro selection of an RNA library. The secondary structure of this RNA contains an internal loop forming an isoalloxazine binding site through non-canonical base pairing. Irradiation with light in presence of FMN induces photocleavage at G.U base pairs outside the aptamer binding site.