A Rapid Method for the Preparation of a One Dimensional Sequence-Overlapping Oligonucleotide Library

R. Bader University of Ulm, Sektion Polymere, D-89069, Ulm

H. Brugger University of Ulm, Abteilung Meß-, Regel- und Mikrotechnik, D-89069, Ulm

M. Hinz University of Ulm, Sektion Polymere, D-89069, Ulm

C. Rembe University of Ulm, Abteilung Meß-, Regel- und Mikrotechnik, D-89069, Ulm

E. P. Hofer University of Ulm, Abteilung Meß-, Regel- und Mikrotechnik, D-89069, Ulm

H. Seliger University of Ulm, Sektion Polymere, D-89069, Ulm

Abstract

A new synthesis modul was constructed to prepare one-dimensional libraries of sequentally overlapping oligonucleotides on surface modified polypropylene tapes. The use of such a library for the diagnostic screening of a point mutation in the regulatory protein gene p53 is described.

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A Rapid Method for the Preparation of a One Dimensional Sequence-Overlapping Oligonucleotide Library

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A Rapid Method for the Preparation of a One Dimensional Sequence-Overlapping Oligonucleotide Library

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