Synthesis of a new Photo-Cross-Linking Nucleoside Analogue Containing an Aryl(Trifluoromethyl)Diazirine Group: Application for Eco RII and Mva I Restriction-Modification Enzymes

Andrey N. Topin Department of Chemistry, Moscow State University, Moscow, 119899, Russia

Oxana M. Gritsenko Department of Chemistry, Moscow State University, Moscow, 119899, Russia

Maxim G. Brevnov Department of Chemistry, Moscow State University, Moscow, 119899, Russia

Elisaveta S. Gromova Department of Chemistry, Moscow State University, Moscow, 119899, Russia

Galina A. Korshunova A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119899, Russia

Abstract

A new photo-cross-linking dU analog, 5-[4-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenyl]-2′-deoxyuridine, was synthesized and incorporated into the recognition site of Eco RII and Mva I restriction-modification enzymes. The resulting base-modified 14-mer substrate was tested for cross-linking to these enzymes. Cross-linking is effected by irradiation of the enzyme-substrate complexs at 366 nm.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Synthesis of a new Photo-Cross-Linking Nucleoside Analogue Containing an Aryl(Trifluoromethyl)Diazirine Group: Application for Eco RII and Mva I Restriction-Modification Enzymes

Information for

Open access

Opportunities

Help and information

Synthesis of a new Photo-Cross-Linking Nucleoside Analogue Containing an Aryl(Trifluoromethyl)Diazirine Group: Application for Eco RII and Mva I Restriction-Modification Enzymes

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature