Extension of the Applicability of δJ-Values for the Configurational Assignment of Diastereomeric Phosphate-Modified Dideoxynucleotides

Abstract

The stereochemical assignment of dinucleoside-S-p-nitrobenzyl-phosphorothioates by NMR spectroscopy is reported. It was found that the method based on the difference of the vicinal phosphorus-carbon coupling constants (δJ =³J_C4′,p-³J_C2′,p) can widely be applied for the determination of the configuration at the pohosphorus atom in phosphate-modified dideoxynucleotides.