![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
Using appropriately designed primers we amplified a 783 bp frag ment of the triple helical portion of human α1-collagen (I) by PCR methods. The expression of this biosynthetic collagen gene and a modified one containing cell adhesion sequences was carried out in a prokaryotic (E. coli) and a eukaryotic (baculovirus) system. The expression products were purified by affinity chromatography.