Oligonucleotide uptake in leukocytes is dependent on extracellular calcium: a hint for the involvement of adhesion molecules?

Gunther Hartmann Medizinische Klinik, Klinikum Innenstadt, University of Munich, Germany

Martin Bidlingmaier Medizinische Klinik, Klinikum Innenstadt, University of Munich, Germany

Bemd Jahrsdörfer Medizinische Klinik, Klinikum Innenstadt, University of Munich, Germany

Anne Krug Medizinische Klinik, Klinikum Innenstadt, University of Munich, Germany

Ulrich Hacker Medizinische Klinik, Klinikum Innenstadt, University of Munich, Germany

Andreas Eigler Medizinische Klinik, Klinikum Innenstadt, University of Munich, Germany

Stefan Endres Medizinische Klinik, Klinikum Innenstadt, University of Munich, Germany

show all

Abstract

An enhanced appreciation of internalization of antisense oligonucleotides will extend possible applications in experimental and therapeutic settings. We found that oligonucleotide incorporation in monocytes, granulocytes and B-lymphocytes but not in T-lymphocytes is strongly dependent on the presence of extracellular calcium, and is inhibited by heparin. Our results support the hypothesis that calcium-dependent adhesion molecules mediate oligonucleotide internalization in leukocytes.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Oligonucleotide uptake in leukocytes is dependent on extracellular calcium: a hint for the involvement of adhesion molecules?

Information for

Open access

Opportunities

Help and information

Oligonucleotide uptake in leukocytes is dependent on extracellular calcium: a hint for the involvement of adhesion molecules?

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature