Influence of P-Chirality of Phosphorothioate Oligonucleotides on the Activity of Amv-Reverse Transcriptase

Abstract

The inhibition of reverse transcriptase by phosphorothioate oligonucleotides of predetermined absolute configuration at each internucleotide centre (PS-oligos) was studied in a cell-free system, composed of an RNA template, a primer oligodeoxynucleotide (PO-oligo) and the AMV or HIV reverse transcriptase. It was found that the non-antisense inhibition of the RT enzymes caused by their direct interaction with PS-oligos depends upon a nucleoside sequence of oligonucleotide as well as upon the absolute configuration at phosphorus atoms of internucleotide phosphorothioate bonds.