Activated DNA- And RNA-Duplexes Covalently Cross-Linked to Regulatory Proteins

Abstract

A novel class of DNA- and RNA duplexes including trisubstituted pyrophosphate internucleotide groups (TSP) in sugar-phosphate backbone was employed in cross-linking to proteins. Substrates including TSP effectively bound covalently to nucleofilic aminoacids (Lys, His) of transcription factors HNF1 and NF-kB and to HIV-1 Tat protein without additional activation at physiological conditions.

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Notes

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