Design and Anti-HIV-1 Activity of Hammerhead and Hairpin Ribozymes Containing a Stable LoopFootnote#
This paper is dedicated to the memory of the late Professor Tsujiaki Hata.

Makoto Koizumi Exploratory Chemistry Research Laboratories, Sankyo Co., LTD, Tokyo, 140, Japan

Yuji Ozawa Biological Research Laboratories, Sankyo Co., LTD, Tokyo, 140, Japan

Ryuichi Yagi Biological Research Laboratories, Sankyo Co., LTD, Tokyo, 140, Japan

Takashi Nishigaki Biological Research Laboratories, Sankyo Co., LTD, Tokyo, 140, Japan

Masakatsu Kaneko Exploratory Chemistry Research Laboratories, Sankyo Co., LTD, Tokyo, 140, Japan

Shin-ichi Oka Department of Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, 108, Japan

Satoshi Kimura Department of Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, 108, Japan; Department of Infection Control and Prevention, the University of Tokyo Hospital, Tokyo, 113, Japan

Aikichi Iwamoto Department of Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, 108, Japan

Yasuo Komatsu Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, 060, Japan

Eiko Ohtsuka Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, 060, Japan

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Abstract

Three ribozymes, a hairpin ribozyme (HR112) and two hammerhead ribozymes (RZ115 and RZ119) containing a 5′C(UUCG)G3′ loop were designed to cleave the U5 region in the long terminal repeat (LTR) of HIV-1 RNA. The t_½ values of chemically synthesized substrates mediated by three ribozymes were measured. The transformed CEM cells possessing these three ribozyme-encoding genes were challenged with a HIV-1_IIIB strain, and two of these three ribozymes, HR112 and RZ119, were shown to possess strong anti-HIV-1 activity.

This paper is dedicated to the memory of the late Professor Tsujiaki Hata.

Notes

This paper is dedicated to the memory of the late Professor Tsujiaki Hata.

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Design and Anti-HIV-1 Activity of Hammerhead and Hairpin Ribozymes Containing a Stable LoopFootnote#
This paper is dedicated to the memory of the late Professor Tsujiaki Hata.

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Design and Anti-HIV-1 Activity of Hammerhead and Hairpin Ribozymes Containing a Stable LoopFootnote#This paper is dedicated to the memory of the late Professor Tsujiaki Hata.

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Design and Anti-HIV-1 Activity of Hammerhead and Hairpin Ribozymes Containing a Stable LoopFootnote#
This paper is dedicated to the memory of the late Professor Tsujiaki Hata.