Repair Kinetics of Abasic Sites in Mammalian Cells Selectively Monitored by the Aldehyde Reactive Probe (ARP)Footnote‡
This paper is dedicated for the late Professor Tsujiaki Hata.

Ayumi Asaeda Department of Veterinary Radiology, School of Veterinary Medicine, College of Agriculture, Osaka Prefecture University, Gakuen-cho, Sakai, Osaka, 593, Japan

Hiroshi Ide Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, 739, Japan

Keizo Tano Research Reactor Institute, Kyoto University, Noda, Kumatori, Osaka, 590-04, Japan

Yasuhiko Takamori Department of Veterinary Radiology, School of Veterinary Medicine, College of Agriculture, Osaka Prefecture University, Gakuen-cho, Sakai, Osaka, 593, Japan

Kihei Kubo Department of Veterinary Radiology, School of Veterinary Medicine, College of Agriculture, Osaka Prefecture University, Gakuen-cho, Sakai, Osaka, 593, Japan

Abstract

Human methylpurine N-glycosylase (MPG) activity was investigated by monitoring abasic (AP) sites resulting from removal of alkylated bases. The amount of AP sites in MMS-treated HeLa cells transiently increased at 3 h, then gradually decreased to 40% at 24 h. The presence of adenine, an inhibitor of AP endonucleases, in the repair incubation of MMS-treated cells induced moderate accumulation of AP sites, suggesting inhibition of the activities of MPG as well as AP endonucleases by adenine metabolites.

This paper is dedicated for the late Professor Tsujiaki Hata.

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This paper is dedicated for the late Professor Tsujiaki Hata.

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Repair Kinetics of Abasic Sites in Mammalian Cells Selectively Monitored by the Aldehyde Reactive Probe (ARP)Footnote‡
This paper is dedicated for the late Professor Tsujiaki Hata.

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Repair Kinetics of Abasic Sites in Mammalian Cells Selectively Monitored by the Aldehyde Reactive Probe (ARP)Footnote‡This paper is dedicated for the late Professor Tsujiaki Hata.

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Repair Kinetics of Abasic Sites in Mammalian Cells Selectively Monitored by the Aldehyde Reactive Probe (ARP)Footnote‡
This paper is dedicated for the late Professor Tsujiaki Hata.