Synthetic Oligonucleotide Combinatorial Libraries. 3. Synthesis of Polyamevonucleosides

Abstract

Synthesis of polyamino-2′-deoxynucleosides was studied. A synthesis of 2′-deoxyadenosine and 2′-deoxyguanosine derivatives carrying a protected spermine moiety at N-6 and N-2 positions respectively is described using unprotected polyamines as substrates. The question of reactivity of primary and secondary amino groups present in polyamines was studied. The evidence that only primary amino groups react with the synthesised precursors was accomplished from experiments with a secondary amine (di-n-butylamine). An approach to analyse properties of polyaminooligonucleotides using their synthetic combinatorial libraries is discussed.