A Pyrene Seco-Pseudonucleoside in Constructing Interaction-Sensitive Fluorescent DNA Probes

Vladimir A. Korshun Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP-7 Moscow, 117871, Russia Fax: E-mail: [email protected])

Konstantin V. Balakin Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP-7 Moscow, 117871, Russia Fax: E-mail: [email protected])

Tatyana S. Proskurina Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP-7 Moscow, 117871, Russia Fax: E-mail: [email protected])

Ilya I. Mikhalev Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP-7 Moscow, 117871, Russia Fax: E-mail: [email protected])

Andrei D. Malakhov Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP-7 Moscow, 117871, Russia Fax: E-mail: [email protected])

Yuri A. Berlin Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP-7 Moscow, 117871, Russia Fax: E-mail: [email protected])

Abstract

Based on 4-(1-pyrenyl)-1, 3-butanediol, a pyrene seco-pseudonucleoside, we prepared a nonnucleotide phosphoramidite reagent and a pyrene-modified LCAA-CPG-support and employed them in solid-phase synthesis of pyrene-labeled oligonucleotides with varying numbers and positions of the pseudonucleoside residues. Changes in the fluorescence intensity and the spectral curve shape upon hybridization were observed that depended on the position(s) of the modified unit(s) in the probe.

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A Pyrene Seco-Pseudonucleoside in Constructing Interaction-Sensitive Fluorescent DNA Probes

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A Pyrene Seco-Pseudonucleoside in Constructing Interaction-Sensitive Fluorescent DNA Probes

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