Eicosanoid Signaling in Insects: from Discovery to Plant Protection

Abstract

Prostaglandins (PGs) and related eicosanoids are signal moieties derived from arachidonic acid and two other C20 polyunsaturated fatty acids. They were discovered in the 1930s in the context of mammalian reproductive physiology; PGs were associated with the prostate gland, hence their name, and they stimulate uterine smooth muscle contraction. Determining PG chemical structures in the early 1960s and demonstrating that they mediate many human pathophysiological events in the 1970s stimulated intensive research over the following decades in universities, governments and the private sector. Interest in the biological significance of PGs in insects arose in the 1970s and 1980s, which opened a new research frontier. PGs act in reproduction, releasing egg-laying behaviors in some species and signaling egg-maturation events in the Drosophila and silk moth models. They act in insect immunity, mediating and coordinating cellular and humoral responses to wounds, infection and invasion. PGs act in ion transport physiology in insect Malpighian tubules and recta. These compounds also mediate physiological trade-offs between insect immunity and reproduction. Finally, they are central players in the molecular ecology of interactions between blood-feeding insects and their vertebrate hosts. Some PG functions are critical at specific, crucial moments in insect lives, moments we consider ‘emergencies,’ such as the immediate reactions to infection. Certain microbial species have keyed into insect PG signaling and they evolved mechanisms to disable insect immune reactions to infection by inhibiting key enzymes in PG biosynthesis. We provide proof-of-principle that RNA interference treatments designed to silence genes in PG signaling disrupts insect immunity. In this review we describe the history, chemistry and biology of PGs. We use this background to argue that because PGs and other eicosanoids act in emergency situations, they are visible targets for development and deployment of novel insect pest management technologies.

Keywords:

• biological control
• eicosanoids
• insect immunity
• novel pest control strategies
• prostaglandins
• sustainable agriculture

ACKNOWLEDGMENTS

We thank Dr. Robeca Rosengaus (Northeastern University) for her detailed review and for raising most interesting questions on the penultimate draft of the manuscript. Thanks, also, to Dennis Grey for inviting this essay. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. This research was also supported by Bio-industry Technology Development Program, Ministry of Agriculture, Food and Rural Affairs, Korea to YK.