Rat PSP94 Inhibits the Growth and Viability of the Rat Adenocarcinoma Cell Line PAIII In Vitro

Peter A. Cadieux Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada; Canadian Research and Development Centre for Probiotics, Lawson Health Research Institute, London, Ontario, Canada

Sebastian A. Mikolajczak Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada

Jonathan Reeves Procyon Biopharma, Inc., Montreal, Quebec, Canada

Craig Strathdee Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada

Gregor Reid Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada; Canadian Research and Development Centre for Probiotics, Lawson Health Research Institute, London, Ontario, Canada; Department of Surgery, St. Joseph's Health Care, London, Ontario, Canada

Chandra J. Panchal Procyon Biopharma, Inc., Montreal, Quebec, Canada

Michael W. Clarke Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada; Procyon Biopharma, Inc., Montreal, Quebec, Canada

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Abstract

Previous studies have shown that human PSP94 can inhibit the growth of prostate cancer cells both in vitro and in vivo. To further validate this potential and investigate the protein within a homologous setting, we examined the effects of rat PSP94 on the growth of the rat prostate adenocarcinoma cell line PAIII in vitro. To generate rat PSP94, we used both a plasmid-based expression system and a recombinant rat PSP molecule. Rat PSP was shown to inhibit the growth and survival of PAIII cells in a dose-dependent manner with > 90 percent reductions in both observed. TUNEL and Annexin-V assays confirmed PAIII cell death to be via apoptosis.

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Rat PSP94 Inhibits the Growth and Viability of the Rat Adenocarcinoma Cell Line PAIII In Vitro

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Rat PSP94 Inhibits the Growth and Viability of the Rat Adenocarcinoma Cell Line PAIII In Vitro

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