The Effect of TGF-β₂ on MMP-2 Production and Activity in Highly Metastatic Human Bladder Carcinoma Cell Line 5637

Ehsan Dehnavi Ministry of Science, Research and Technology, National Institute of Genetic Engineering and Biotechnology; Khatam University

Zahra-Soheila Soheili Ministry of Science, Research and Technology, National Institute of Genetic Engineering and Biotechnology

Shahram Samiei Iranian Blood Transfusion Organization Research Center

Zahra Ataei Iranian Blood Transfusion Organization Research Center

Hajar Aryan Payame-noor University of Tehran

Abstract

Transforming growth factor–beta (TGF-β) superfamily regulates matrix metalloproteinases (MMP), which intrinsically regulate various cell behaviors leading to metastasis. We investigated the effect of TGF-β₂ on MMP-2 regulation in human bladder carcinoma cell line 5637. Zymography, ELISA, and real-time polymerase chain reaction revealed that TGF-β₂ stimulated MMP-2 production, but the transcription of its gene remained unchanged. Wortmannin could not inhibit MMP-2 secretion and activity and conversely the amount of the protein and its enzymatic activity were increased. These data suggest that TGF-β₂ increased MMP-2 at the posttranscriptional level and this upregulation was independent of phosphatidylinositol 3-kinase signaling pathway.

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The Effect of TGF-β₂ on MMP-2 Production and Activity in Highly Metastatic Human Bladder Carcinoma Cell Line 5637

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The Effect of TGF-β2 on MMP-2 Production and Activity in Highly Metastatic Human Bladder Carcinoma Cell Line 5637

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The Effect of TGF-β₂ on MMP-2 Production and Activity in Highly Metastatic Human Bladder Carcinoma Cell Line 5637